Abstract: Over the past decade biological small angle X-ray scattering (SAXS) has made a growing entry into industrial pharmaceutical drug development. Driven by the need for better understanding of the structure, stability and behavior of biologics, drug carrier systems and other complex molecules in many different scenarios and by the large technological advances in home laboratory SAXS and the maturity of analysis tools, SAXS is now making an impact in every stage of the drug development process. In this short oveview talk on SAXS in the pharmaceutical industry we will highlight examples of where SAXS is being used at different stages in industrial drug development and the capabilities of modern SAXS laboratory equipment.

Abstract: Many of Xenocs customers use their home laboratory SAXS instrument for biological small angle X-ray scattering (SAXS). This review presentation will cover several examples from litterature of pharmaceutical relevant topics done by Xenocs customers on their instruments. The topics include, amog other, vesicles, emulsions, drug carrier miscelles and nano particles.

Abstract: Small angle X-ray scattering is a powerful technique for studying proteins and other macromolecules in solution. Recent progress in SAXS instrumentation and data analysis methods allows SAXS to address increasingly complicated structural biology questions. These include evaluation of the overall geometrical parameters and low-resolution shape reconstruction in the absence of a priori structural information as well as structure validation, identification of biologically active oligomers and rigid body modeling of multisubunit complexes when high resolution structures of the components are available. Monodispersity of the sample is a crucial prerequisite for reliable data analysis; however despite cautious sample preparation some proteins remain polydisperse or even unfolded. Applicability of various tools from the ATSAS package for structural analysis of monodisperse and polydisperse systems will be discussed.

Abstract: Monoclonal antibodies (mAbs) are the largest class of biopharmaceuticals drugs that are widely used in clinical diagnostics and in protein-drug development. mAbs are being used at high concentrations to achieve the desired therapeutic doses. However, achieving a stable formulation of mAbs at efficacious concentration increases non-specific protein-protein interactions that could lead to self-association and aggregation. Understanding of rationale behind mAbs stability in concentrated formulations pose substantial challenges for biophysical techniques as most of them are performed in dilute solutions. Small-angle X-ray scattering (SAXS) has emerged as an integrative technique to study the behavior of mAbs and other protein-drugs over a broad of concentration and molecular weight. My talk will briefly cover the use of SAXS to study the physical stability of mAbs and mAbs-protein interactions.

Abstract: Lipid membranes exhibit essential mechanical and physiological properties to keep the form of life intact for their functioning. Lipids work towards storage of energy, regulation of moisture, temperature and pressure, transporting and conserving essential nutrients and cholesterol and inside body etc. Model phospholipids are often used for studying the impact of physical parameters such as temperature and pressure on its interaction with of membrane active peptides and drugs. The presentation will discuss on how structural information using small angle scattering works in tandem with the spectroscopic techniques for peptides to resolve interactions between lipids, proteins and drugs.